UACES Facebook Nematodes in crop production in Arkansas - Arkansas Nematodes
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Arkansas Nematode Diagnostic Laboratory

Plant-parasitic nematodes are an economically important pest that affect many row crops, horticultural crops, golf courses, and trees and shrubs in Arkansas. Nematodes are host-specific organisms and the strategies for management of the species will vary by crop. Chemical control options for nematodes are limited so often rotation to a non-host crop is the best solution. The Arkansas Nematode Diagnostic Laboratory offers bioassay and quantification services for the following crops:

  • Cotton, Soybean, Corn, and other Row Crops (Root-knot, Soybean cyst, Reniform, Lesion, etc)
  • Rice (White tip nematode pest-free certification for export)
  • Cedar and Pine (Pinewood nematode pest-free certification for export)
  • Turf (Lawns and golf courses)
  • Horticultural crops and shrubs (Commercial crops and home garden and landscape, pest-free certification for nursery stock)
  • Forage crops
  • Custom assays are available (Call for info 870-899-4199)
  • Out-of-state samples are accepted per APHIS regulations. Permit must be printed and included with samples.  Click here to see our permit.

  pinewood nematode damage in pine treesnematode damage on a golf coursewhite discoloration to tips of rice caused by white tip nematodeMargie Miller runs the semi-automatic elutriator at the ANDL

Photo 1 (Top left): Pine trees near Corning Arkansas with Bursaphelenchus xylophilus (Pinewood nematode)
Photo 2 (Top right):  Golf course with nematode damage to putting green
Photo 3 (Bottom left):  Rice plot with Aphelenchoides besseyi (White tip nematode)
Photo 4 (Bottom right):  Margie Miller, Agriculture Lab Technician, operates the semi-automatic elutriator for processing nematode samples

Services and Fees

 A general soil assay allows for detection of all vermiform nematodes within a row crop, horticultural crop or turf grass sample.  


Sampling Instructions:
(for row crops, annual ornamentals, hemp, and vegetables)
Use tube-type soil probe
Insert 6-8" deep in soil near root zone
Collect 10-20 cores in zig zag pattern
Mix and place 1 pint of soil in plastic bag, secure with wire tag, label with pencil

(for fruit and nut trees, shrubs, and trees)
Use tube-type probe
Collect several cores near root zones of affected plants
If drip lines are present, remove top inch of soil and collect samples that include feeder roots
One sample should consist of 10 cores 8" deep or 20 cores from multiple plants mixed together
Place one pint of sampled soil in plastic bag, secure with wire tag, label with pencil

(for turf grass: golf courses, athletic fields, lawns, forages)
Use tube-type probe
Insert 3-4" in declining (but not completely dead) turf area
Collect 10-20 cores
Mix thoroughly and place 1 pint of soil in plastic bag, secure with wire tag, label with pencil

Shipping Instructions:
Place samples in empty ice chest if collecting many and weather is hot
Do not store in hot areas for long periods
Fill out a submission form for each sample
Do not put forms in bags
Place no more than 10 samples per box
Print and place completed submission forms in box on top of samples
Ship to ANDL early in the week for best results

A soybean cyst nematode assay is done for detecting vermiform and eggs.

An additional race assay can be done for the purpose of identifying the race of the cyst nematode if population exceeds threshold.  Allow 50 extra days for testing.  If population does not exceed threshold, you will only be charged for general assay.

Sampling Instructions:
Use tube-type soil probe
Insert 6-8" deep in soil near root zone
Collect 10-20 cores in zig zag pattern
Mix and place 1 pint of soil in plastic bag, secure with wire tag, label with pencil

Shipping Instructions:
Place samples in empty ice chest if collecting many and weather is hot
Do not store in hot areas for long periods
Fill out a submission form for each sample
Do not put forms in bags
Place no more than 10 samples per box
Print and place completed submission forms in box on top of samples
Ship to ANDL early in the week for best results

 A nursery assay must be done for ornamentals crossing state lines and will detect all quarantined nematodes.  

Sampling Instructions:
Both root and soil samples may be necessary
Contact the Arkansas State Plant Board for details

Shipping Instructions:
Place samples in a crush-proof container.

A garlic assay is to detect Ditylenchus in garlic cloves.

Submit 10 heads of healthy and 10 heads of suspect garlic as your sample. Place in crush proof container for shipping (box).

A rice white tip nematode assay is necessary for shipments of rice being exported to countries where this nematode is quarantined.  Individuals wanting a rice white tip assay for non-exported rice may submit the grain samples to the ArNDL for a $25 fee.  

Sampling Instructions:
Contact the Arkansas State Plant Board for proper sampling and handling instructions.

Shipping Instructions:
Overnight delivery is preferred.

A pinewood nematode assay is required for coniferous products being exported to countries where the nematode is quarantined.  

Sampling Instructions:
Contact the Arkansas State Plant Board for proper sampling and handling instructions.

Shipping Instructions:
Ship to ANDL early in the week for best results. 

 

Online Sample Submission Using The Distance Diagnostics Through Digital Imaging Service (DDDI)

Requests for nematode analysis are submitted electronically through the University of Arkansas Distance Diagnostics System (DDDI). Click the link to begin the submission process: http://www.dddi.org/ua/ A username and password are required to access the system. If this is your first time to use the system, click on ‘Request An Account’ to obtain a username and password. Your username and password will be e-mailed to you. This is the same system used for the Plant Health Clinic, and you may use the same username and password if you already have one. Once you have logged in to the DDDI, click on Nematode Sampling Submission in the upper left-hand corner underneath Submit a Sample. After you have completed your submission, print a copy of the request and include in the box with your sample. If you need assistance completing the submission process, please contact Cathy Howard at 870-899-4199. 

Traditional Sample Submission Through Your County Extension Agent

Your county agent can provide forms to be completed for each of your nematode samples.  These forms are placed on top of bagged samples in the mailing box.  Your county agent will be able to guide you through the process of submitting nematode samples through your county office or you may contact Cathy Howard at 870-899-4199, nemalab@uaex.edu for more information.


 

Need to consult with a nematology or plant pathology expert?  

Contact Amanda Greer, Instructor - Plant Pathology at agreer@uaex.edu.

Watch the nematode extraction process

Our Team

Amanda Greer  
Cathy Howard Research Technologist
 
Margie Miller Agriculture Lab Technician

Amanda Greer
Diagnostician/Instructor - Plant Pathology

Ph. (870) 777-9702 ext. 119
Email: agreer@uaex.edu

 

Cathy Howard
Research Technologist

Ph. (870) 777-9702 ext. 128
Email: choward@uaex.edu

 

Margie Miller
Agricultural Lab Technician

Ph. (870) 777-9702 ext. 127
Email: mmiller@uaex.edu

 

Photo coming soon sign

 

James Lamb

 

JD Barham

Tammy Woodruff
Service Assistant II


Email: thickey@uark.edu
 

James Lamb
Seasonal Intern


Email: jlamb@uaex.edu

 

JD Barham
Program Technician


Email: jbarham@uaex.edu

 

Additional Resources

 

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