UACES Facebook Nematodes in crop production in Arkansas - Arkansas Nematodes

Arkansas Nematode Diagnostic Laboratory

Plant parasitic nematodes are an economically important pest that affect many row crops, horticultural crops, golf courses, and trees and shrubs in Arkansas. Nematodes are host-specific organisms and the strategies for management of the species will vary by crop.  Chemical control options for nematodes are limited so often crop rotation to a non-host is the best solution.  The Arkansas Nematode Diagnostic Laboratory offers bioassay and quantification services for the following crops:

  • Cotton, Soybean and Corn (Root-knot nematode, Soybean cyst nematode, Reniform nematode)
  • Rice (White tip nematode pest-free certification for export)
  • Cedar and Pine (Pinewood nematode pest-free certification for export)
  • Turf (Lawns and golf courses)
  • Horticultural crops and shrubs (Commercial crops and home garden and landscape, pest-free certification for nursery stock)
  • Forage crops
  • Custom assays are available
  • Out-of-state samples are accepted per APHIS regulations.  Click here to see our permit.

  pinewood nematode damage in pine treesnematode damage on a golf coursewhite discoloration to tips of rice caused by white tip nematodeMargie Miller runs the semi-automatic elutriator at the ANDL

Photo 1 (Top left): Pine trees near Corning Arkansas with Bursaphelenchus xylophilus (Pinewood nematode)
Photo 2 (Top right):  Golf course with nematode damage to putting green
Photo 3 (Bottom left):  Rice plot with Aphelenchoides besseyi (White tip nematode)
Photo 4 (Bottom right):  Margie Miller, Agriculture Lab Technician, operates the semi-automatic elutriator for processing nematode samples

Services and Fees

  • General Assay for Row Crops, Horticultural Crops, and Turf - $10

    A general assay allows for detection of all nematodes within a row crop, horticultural crop or turf grass sample.  

    Sampling Instructions:
    (for row crops, annual ornamentals, and vegetables)
    Use tube-type soil probe
    Insert 6-8" deep in soil near root zone
    Collect 10-20 cores in zig zag pattern
    Mix and place 1 pt. in plastic bag, secure with wire tag, label with pencil

    (for fruit and nut trees, shrubs, and trees)
    Use tube-type probe
    Collect several cores near root zones of affected plants
    If drip lines are present, remove top inch of soil and collect samples that include feeder roots
    One sample should consist of 10 cores 8" deep or 20 cores from multiple plants mixed together
    Place one pint of sampled soil in plastic bag, secure with wire tag, label with pencil

    (for turf grass-golf courses, athletic fields, lawns)
    Use tube-type probe
    Insert 3-4" in declining turf area
    Collect 10-20 cores
    Mix thoroughly and place 1 pt. of soil in plastic bag, secure with wire tag, label with pencil

    Shipping Instructions:
    Place samples in empty ice chest if collecting many and weather is hot
    Do not store in hot areas for long periods
    Fill out a submission form for each sample
    Do not put forms in bags
    Place no more than 10 samples per box
    Print and place completed submission forms in box on top of samples
    Ship to ANDL early in the week for best results

  • Soybean Cyst Nematode Assay - $20

    A soybean cyst nematode assay is done for the purpose of identifying the race of the cyst nematode.  A race assay will be done if population exceeds threshold.  Allow 40-50 extra days for testing.  If population does not exceed threshold, you will only be charged for general assay.

    Sampling Instructions:
    Use tube-type soil probe
    Insert 6-8" deep in soil near root zone
    Collect 10-20 cores in zig zag pattern
    Mix and place 1 pt. in plastic bag, secure with wire tag, label with pencil

    Shipping Instructions:
    Place samples in empty ice chest if collecting many and weather is hot
    Do not store in hot areas for long periods
    Fill out a submission form for each sample
    Do not put forms in bags
    Place no more than 10 samples per box
    Print and place completed submission forms in box on top of samples
    Ship to ANDL early in the week for best results

  • Nursery Assay - $25

    A nursery assay must be done for ornamentals crossing state lines and will detect all quarantined nematodes.  

    Sampling Instructions:
    Both root and soil samples may be necessary
    Contact the Arkansas State Plant Board for details

    Shipping Instructions:
    Place samples in a crush-proof container.

  • White Tip Nematode Assay for Exported Rice - $75

    A rice white tip nematode assay is necessary for shipments of rice being exported to countries where this nematode is quarantined.  Individuals wanting a rice white tip assay for non-exported rice may submit the grain samples to the ANDL for a $25 fee.  

    Sampling Instructions:
    Contact the Arkansas State Plant Board for proper sampling and handling instructions.

    Shipping Instructions:
    Overnight delivery is preferred.  

  • Pinewood Nematode Assay for Exported Pine and Cedar - $25

    A pinewood nematode assay is required for coniferous products being exported to countries where the nematode is quarantined.  

    Sampling Instructions:
    Contact the Arkansas State Plant Board for proper sampling and handling instructions.

    Shipping Instructions:
    Ship to ANDL early in the week for best results. 

Online Sample Submission Using The Distance Diagnostics Through Digital Imaging Service (DDDI)

Requests for nematode analysis are submitted electronically through the University of Arkansas Distance Diagnostics System (DDDI). Click the link to begin the submission process: http://www.dddi.org/ua/ A username and password are required to access the system. If this is your first time to use the system, click on Request An Account to obtain a username and password. Your username and password will be e-mailed to you. This is the same system used for the Plant Health Clinic, and you may use the same username and password if you already have one. Once you have logged in to the DDDI, click on Nematode Sampling Submission in the upper lefthand corner underneath Submit a Sample. After you have completed your submission, print a copy of the request and include in the box with your sample. If you need assistance completing the submission process, please contact Cathy Howard at 870-777-9702, ext. 128. 

Traditional Sample Submission Through Your County Extension Agent

Your county agent can provide forms to be completed for each of your nematode samples.  These forms are placed on top of bagged samples in the mailing box.  Your county agent will be able to guide you through the process of submitting nematode samples through his or her office or you may contact Cathy Howard at 870-777-9702, ext. 128, for more information.


 

Have questions only a nematologist can answer?  

Contact Dr. Terry Kirkpatrick - Professor/Extension Plant Pathologist at (870) 777-9702 ext. 111 or tkirkpatrick@uaex.edu.

Watch the nematode extraction process

Our Team

Ronnie Bateman-Program Associate

Ronnie Bateman


Diagnostician/Director-ANDL
Program Associate-Plant Pathology

Phone:  (870) 777-9702 ext. 119
Email:  rbateman@uaex.edu

 
 
Cathy Howard-Research Associate

Cathy Howard


Research Associate

Phone:  (870) 777-9702 ext. 128
Email:  choward@uaex.edu

 
 
Katie Sullivan, Program Technician

Katie Sullivan

Diagnostician
Program Technician

Phone:  (870) 777-9702 ext. 119
Email:  kamccrar@uark.edu

 

 

 

Margie Miller-Agricultural Lab Technician

Margie Miller


Agricultural Lab Technician

Phone:  (870) 777-9702 ext. 12
Email:  mmiller@uaex.edu

 
 

Additional Resources