UACES Facebook Fellowship Recipient 2012: Kimberly Cochran - November 2012

Fellowship Recipient 2012: Kimberly Cochran - November 2012

You Tube - Link to watch video on You Tube.Link to transcript

Audio/Video Script:

My name is Kimberly Cochran and we’re in Fayetteville, Arkansas, at the University of Arkansas and I’m a Ph.D. candidate here in the plant pathology department. [Video shows Kimberly working in a lab.]

[University of Arkansas Division of Agriculture Research and Extension University of Arkansas System. Arkansas Soybean Promotion Board. Kimberly Cochran, Ph.D. candidate of Plant Sciences at the University of Arkansas 2012 A S P B Fellowship Recipient]

[Kimberly Cochran] The Soybean Board has been kind enough to fund all of my research and I’m able to do larger projects like the 454 sequencing that I wouldn’t be able to otherwise because it is so expensive. But it might offer insight into the seed microflora and how it’s affecting the quality and vigor, which impacts the growers and their emergence problems in the field. [Picture of colletotrichum fungus under a microscrope.]

Every year, we grow our seed at Kibler, at the research station, [pictures of soybean seed] and then we harvest the seed by hand and then it’s taken here and cleaned, which means we pick out all of the debris and dead insects and things like that. And after that, it’s stored in bags until I can test it, [video shows Kimberly running tests in the lab] performing various quality and vigor tests including standard germination, accelerated aging, Estes, which is the seed imaging system, electrical conductivity testing, and then after that, I do what’s called microflora plating where I take Petri plates and place seeds on the Petri plates and I look at what organisms grow off of those seeds. Then I record that, store some isolates to keep a representation and then finally, I’m in the process now of selecting some seed lots and then extracting DNA from those for fungal and bacterial organisms and then I finally get to do the molecular techniques with that.  So, in a nutshell, that is three years worth of work. [Video shows Petri dishes with sprouted soybeans; seeds in dishes with phomopsis fungi and dishes with fusarium graminearum fungi.]

[Kimberly Cochran] I’d like to learn more about the microflora composition and how it affects seed vigor, not just quality vigor, but how seed perform under stress. Quality is how seed perform under ideal conditions. So I want to see how microflora affects seed performing under stress and that might help explain why some farmers have more of a problem with stands in the field. [Video shows seeds in a dish with cercospora kikuchii fungi, seeds in a dish with colletotrichum fungi, and seeds in a dish with cercospora fungi.]

[www.uaex.edu. University of Arkansas Division of Agriculture Research and Extension University of Arkansas System. Arkansas Soybean Promotion Board]

 

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