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Aquaculture/Fisheries Center Research Highlights
ARS Aquaculture Systems Research Unit
 
  1. A Unit to Continuously Monitor Dissolved Oxygen and Temperature at User-Selected Depths and Locations in Culture Ponds
    Philip R. Pearson, Bartholomew W. Green

    A 2004 field study conducted during actual channel catfish Ictalurus punctatus harvests and a small-scale research study conducted in 2005, required continuous measurement of dissolved oxygen concentration and temperature at two depths in the water column. The on-farm study required data collection in grading nets loaded with high densities of channel catfish, whereas the small-scale study was conducted in 0.1-acre channel catfish research ponds. Commercially available monitoring units did not meet study requirements, so a free-floating data collection unit comprised of a combination of state-of-the-art sensor and datalogger technology and readily available materials was developed. The data collection units proved durable under conditions existing in both loaded grading nets and in small research ponds.
     
  2. A Calculator for Estimating Oxygen Consumption By Channel Catfish
    Philip R. Pearson, Rachel V. Beecham

    A review of scientific literature concerning channel catfish Ictalurus punctatus respiration resulted in development of a Microsoft Excel© spreadsheet for estimating the volume of oxygen consumed by a given channel catfish biomass. Channel catfish farmers and live haulers using diffused oxygen aeration systems can use computed estimates of oxygen consumption as a guide for setting flow rates for diffusers placed in grading nets and haul tanks, respectfully. Entry of eight variables into the spreadsheet provides estimates of oxygen gas consumption with respect to time and biomass, the liquid oxygen equivalent of that volume, liquid oxygen expense, and the ratio of liquid oxygen expense to gross revenue.
     
  3. Impact of Horizontal or Vertical Circulation on Water Quality, Plankton, and Catfish Production
    Bartholomew Green, Peter Perschbacher, Kevin Schrader

    The present study was conducted to evaluate the effect of a horizontal discharge or an up-welling vertical discharge circulator on water quality, plankton and channel catfish production. Twelve 0.25-acre earthen ponds were used for this completely randomized design study. Four ponds each were equipped with a low-revolutions-per-minute (approximately 6 RPM) horizontal discharge circulator or an up-welling vertical discharge circulator. Circulators operated continuously. All ponds were equipped with an electric paddlewheel aerator (0.373 kW) that was activated when pond DO concentration was 4.0 mg/L or less. Control ponds were equipped with only the paddlewheel aerator. Ponds were stocked with stocker catfish at 4,567 lb/acre; mean initial stocker weight was 0.72 lb/fish. Fingerling catfish (32.8 lb/1,000) were understocked at 6,000/acre. Ponds were stocked in late January to early February. Fish were fed a 32% protein floating extruded feed daily to apparent satiation. Ponds were harvested during the last week of October. Green algae predominated in all treatments ponds through mid-June, after which blue-green algae predominated until harvest. Chlorophyll a concentrations were similar among treatments, and averaged 240.3, 197.2, and 194.7 mg/m3 for the control, horizontal and vertical circulation treatments, respectively. Off-flavor algae were present in treatment ponds from mid-July through early September. No treatment differences were detected among water quality variable means. However, temperature and dissolved oxygen concentration profiles were uniform to the 30-in depth in circulated ponds. Thus, excess photosynthetically produced dissolved oxygen was retained in circulated ponds. Net catfish yield was similar among treatments and averaged 7,484 lb/acre, of which 86% exceeded 1.25 lb average weight. Mean individual weight at harvest among treatments was 1.99 lb. At harvest, fish from all treatments were classified as being off-flavor. Pond circulation impacted positively pond temperature and dissolved oxygen, but had no significant impact on fish production, plankton, or water quality variables.
     
  4. Effect of Stocking Rate on Growth, Survival and Yield of Channel x Blue Hybrid Catfish in a Microbially Based Production System
    Bartholomew Green

    The effect of stocking rate on growth, survival, and yield of channel x blue hybrid catfish reared in a microbially based production system was evaluated in this study. Nine 450 ft2 x 30” deep, HDPE-lined divided raceways were used for this study. Each raceway was equipped with a ½-hp electric paddlewheel aerator that operated continuously. Stocker channel x blue hybrid catfish (186 lb/1,000) were stocked into raceways at 25, 50, 75, 100, 125, 150, 175, 200, and 225 fish/raceway. Fish were fed a 32% protein feed. Additional carbon, as wheat flour, was added daily to each raceway based on feed additions beginning in July. Water quality variables were measured at weekly intervals. Raceways were harvested after 188 days. At harvest, mean individual fish weight ranged from 0.74 – 1.19 lb/fish. Mean individual fish weight decreased linearly with increased stocking rate. Fish survival ranged from 60 – 80%, and averaged 70%. The biomass of fish at harvest increased linearly from 0.01 – 0.08 lb/ft3. Dense phytoplankton blooms formed in the raceways as indicated by chlorophyll a concentrations ranged from 1,000 – 4,000 mg/m3. Microbial action maintained low concentrations of total ammonia nitrogen and nitrite nitrogen in all raceways, and resulted in concentrations of nitrate nitrogen that ranged from 20 – 90 mg/L NO3-N at the end of the experiment. The results of this study indicate that hybrid catfish can be grown in a microbially based production system in raceways. Mean weight at harvest was inversely proportional to stocking rate, while final biomass was directly proportional to stocking rate. Further testing and refinement of this system is on-going.



 
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